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Lipidomics & Metabolomics

Rates

VLMC Analysis

USF Affiliated

CMMB Affiliated

External Non-profit Organizations

TA: Sphingolipids

$20.00

$15.00

$30.50

TA: Eicosanoids

$28.00

$21.00

$42.70

TA: Steroid Hormones

$20.00

$15.00

$30.50

TA: Lysophosphatidic Acid

$22.00

$16.50

$33.55

TA: Vitamin D

$20.00

$15.00

$30.50

TA: Phospholipids

$22.00

$16.50

$33.55

TA: Cholesterols

$20.00

$15.00

$30.50

TA: Cardiolipins

$20.00

$15.00

$30.50

UTA: Systems Level Lipidomics

$65.00

$48.75

N/A

UTA: Systems Level Metabolomics

$90.00

$67.50

N/A

TA: targeted, quantitative analysis; UTA: untargeted, qualitative analysis

Before submitting samples, please contact Daniel Stephenson stephensond@usf.edu to set up appropriate logistics. Some lipids are unstable and require immediate analysis. Our goal is to provide you with the best data possible! All data will be delivered to the submitting individual and laboratory PI as an easy-to-read Excel file.

Eicosanoid analysis: From serum, plasma, tissues, cells, cell media (dye-free preferred), lavages, etc…, we concentrate via a SPE column followed by the quantitative, targeted analysis of 30 analytes using deuterated standards.

These include: 6-keto Prostaglandin F1α, 8-iso Prostaglandin F2α, Thromboxane B2, 5-iPF2α-VI, Prostaglandin E2, Prostaglandin F2α, Prostaglandin D2, Resolvin D2, Prostaglandin E1, Resolvin D1, Lipoxin A4, Prostaglandin A2, Leukotriene B4, Leukotriene C4, Leukotriene E4, Leukotriene D4, (±)14,15-DHET, 15-deoxy-Δ12,14-Prostaglandin J2, (±)11,12-DHET, (±)8,9-DHET, 20-HETE, 15 HETE, 12 HETE, (±)14(15)-EET, 5 HETE, (±)8(9)-EET, Eicosapentaenoic Acid, Docosahexaenoic Acid, Arachidonic Acid, and Dihomo-Gamma-Linolenic Acid

Coming soon: We will also be offering a semi-quantitative, “relative change” analysis, which will include: Resolvin E1, maresin 1, Prostaglandin D3, Prostaglandin E3, Resolvin D5, Resolvin D3, maresin, 2,10(S),17(S)-DiHDoHE (PDX), Prostaglandin J2. As 13C and deuterated standards are not available for these analytes, we use the closely related PGE2, PGE3, and Resolvin D2 internal standards (deuterated). These data are considered quantitative for identifying fold-changes between provided samples, but the actual quantitation pmols/mL, etc… are considered semi-quantitative. As deuterated or 13C standards become available for these compounds, they will be added to our full quantitative analysis package for no additional charge.

Sphingolipid analysis: From serum, plasma, tissues, cells, cell media (dye-free preferred), lavages, etc…, we extract samples using a modified Bligh-Dyer followed by the quantitative, targeted analysis of:

Sphingosine

Sphinganine

Sphingosine-1-phosphate

Sphinganine-1-phosphate

Ceramide (N-acyl chain lengths = C14:0, C16:0, C18:1, C18:0, C20:0, C22:0, C24:1, C24:0, C26:1, C26:0)

Monohexosylceramide (N-acyl chain lengths = C14:0, C16:0, C18:1, C18:0, C20:0, C22:0, C24:1, C24:0, C26:1, C26:0)

Sphingomyelin (N-acyl chain lengths = C14:0, C16:0, C18:1, C18:0, C20:0, C22:0, C24:1, C24:0, C26:1, C26:0)

Ceramide 1-Phosphate (N-acyl chain lengths = C14:0, C16:0, C18:1, C18:0, C20:0, C22:0, C24:1, C24:0, C26:1, C26:0) (Of note: C1Ps are labile and usually require “fresh” samples for appropriate detection and quantitation).

Coming soon: As soon as scientific/biological validation is complete, we will be offering deoxysphingolipids as well.

Steroid analysis: We extract samples using a modified Bligh-Dyer followed by the quantitative, targeted analysis of:

Dehydroepiandrosterone, Androstenedione, Testosterone, Estradiol, 2-Methoxyestradiol, Dihydrotestosterone, Cortisol, Progesterone, and Corticosterone. 

Cardiolipin analysis: We extract samples using a modified Bligh Dyer followed by the quantitative analysis of the following: (C14:0)4, (C18:2)3(C18:3)1, (C18:2)4, (C18:2)3(C18:1)1, (C18:2)2(C18:1)2, (C18:2)3(C18:0)1, (C18:2)3(C22:6)1, (C18:2)2(C18:1)1(C22:6)1, and (C18:2)2(C22:6)2.

Coming soon: As soon as scientific/biological validation is complete, we will be offering certain oxidized cardiolipin species as well.

Lysophosphatidic acid analysis: We extract samples using a modified Bligh-Dyer followed by the quantitative, targeted analysis of: (16:0, 18:2, 18:1, 18:0, and 20:4).

Systems level, untargeted lipid analysis: We extract samples using a modified Bligh-Dyer followed by the quantitative analysis of multiple lipid classes e.g; (Glycerophospholipids, Sphingolipids, Glycerolipids, Sterols, & Fatty Acids).  Plasma analysis provides approximately 2000 positive and 2000 negative analytes. 

Coming Soon

Phospholipid Analysis: We extract samples using a modified Bligh-Dyer followed by the quantitative, targeted analysis of:

PE’s (16:0-20:4 PE, 16:0-22:6 PE, 18:0-20:4 PE, 18:0-22:6 PE, 16:0 Lyso PE, 18:0 Lyso PE, EPA, DHA, & AA)

PC’s (16:0-20:4 PC, 16:0-22:6 PC, 16:0 Lyso PC, 18:0-20:4 PC, 18:0-22:6 PC, & 18:0 Lyso PC)

Urine Metabolites: We concentrate via a SPE column followed by the quantitative, targeted analysis of: (2,3-dinor-6-keto PGF1alpha-d9, 2,3-dinor TXB2-d9, 11-dehydro TXB2-d4, 2,3-dinor-6-keto PGF1alpha, 11-dehydro-2,3-dinor TXB2, 2,3-dinor TXB2, & 11-dehydro TXB2).

These will be added soon to the Eicosanoid Analysis at no additional charge.

Vitamin D Analysis: We extract samples using a LLE method followed by the quantitative, targeted analysis of: (Vitamin D3, 25-hydroxyvitamin D3, 3-epi-25-hydroxyvitamin D3, & 24R,25-dihydroxyvitamin D3).

Cholesterol & Cholesterol Esters: In process

Citation

All publications that include results, services or products generated by USF CMMB Core Facility must include the following acknowledgement in the manuscript:

"Services and products in support of the research project were generated by the USF CMMB Core Facility, supported, in part, with funding from NIH-NCI Cancer Center Support Grant  P30 CA016059."

PubMed Central (PMC) ID numbers are required for those publications that use CCSG-supported shared resources. All of Massey Cancer Center's shared resources are supported by the CCSG. Information to assist you with this process can be found here.