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Mass Spectrometry

LTQ Ion Trap

  • Unparalleled sensitivity and unmatched MSn spectral quality
  • Pulsed Q Dissociation (PQD) for generating more fragments and extending the low mass range
  • Electron Transfer Dissociation (ETD) option provides sequence information not available from conventional methods
  • High Resolution Isolation (HRI) enables isolation of isobaric mass peaks for cleaner MSn spectra
  • Ultra-fast polarity switching for high sensitivity analysis of unknowns
  • Automated Data Dependent neutral loss for PTM and biotransformation analyses
  • Excellent charge state determinations with Ultra ZoomScan*
  • Upgradeable to LTQ Orbitrap XL accurate mass technology

Compatible with:

  • Accela High-Speed LC System (IQLAAAGABHFAAAMAAG)
  • Surveyor Plus HPLC System (IQLAAAGAAOFAFHMALP)
  • LTQ FT Ultra Hybrid Mass Spectrometer (IQLAAEGAAPFADAMAII)
  • LTQ Orbitrap Hybrid Mass Spectrometers ( IQLAAEGAAPFADBMAIJ)
  • ETD Fragmentation, Electron Transfer Dissociation (IQLAAEGABBFACCMAFJ)
  • LCquan 2.5.5 ( SID-LCQUAN25)
  • Mass Frontier Spectral Interpretation Software (IQLAAEGABOFAGUMZZZ)
  • MetWorks Automated Metabolite Identification Software (IQLAAEGABOFAGTMZZZ)
  • PEAKS Studio 4.0 (SID-PEAKS40)
  • ProMass Deconvolution 2.5 (OPTON-20875)
  • Pulsed-Q Dissociation (PQD) (IQLAAEGABBFAGQMZZZ)
  • SIEVE Automated Label-Free Differential Expression Software (INQSOF012/IQLAAEGABSFAHSMAPV)

Compliance: Manufactured in an ISO 9001:2000 certified facility.

Recommended for:
  • Protein identification and quantitative differential expression analysis
  • Biomarker studies and PTM identification
  • Metabolite identification and quantification
  • Tissue imaging
  • High-speed LC methods
Hardware Features

Ion Max* API Source

  • Enhanced sensitivity and ruggedness
  • Sweep Gas* reduces chemical noise
  • 60° interchangeable ion probe orientation
  • Removable metal ion capillary tube provides vent-free maintenance

Transfer Ion Optics

  • Advanced ion guides
  • High stability and ion transmission efficiency

2D Linear Quadrupole Ion Trap Mass Spectrometer

  • Optimized analyzer dimensions
  • Regulated helium flow for stable performance
  • Automatic system calibration
  • High-efficiency radial ion ejection

Vacuum System

  • Differentially-pumped vacuum system to 10-5 Torr
  • Split-flow turbomolecular pump controlling vacuum in three regions
  • Dual rotary vacuum pump configuration
  • High-vacuum aluminum analyzer chamber

Detection System

  • Proprietary dual conversion dynode detector
  • Two off-axis continuous dynode electron multipliers with extended dynamic range
  • Digital electronic noise discrimination

Integrated Divert Valve

  • Fully-automated data system control enables user to divert the solvent front, gradient end point and any other portion of the HPLC run to waste
  • User-definable default state of the valve, either "to waste" or “to source”

Integrated Syringe Pump

  • Syringe Pump allows automated infusion under data system control
  • Options
    • ESI probe compatible with liquid flowrates of <1µL/min to 1mL/min, without splitting
    • APCI source compatible with liquid flowrates of 50µL/min to 2mL/min, without splitting
    • APCI/APPI source compatible with liquid flowrates of 50µL/min to 2mL/min, without splitting
    • Electron Transfer Dissociation (ETD) enabled
    • vMALDI* ion source combines the speed of MALDI with the sensitivity and selectivity of MS/MS
    • Nanospray source supports static packed tip and dynamic nanospray experiments, compatible with liquid flowrates of 50nL/min† to 2µL/min
    • Metal needle option for high- and low-flow analyses

    †Lower limit is dependent on gauge of needle used

    Software Features

    Data System

    • Xcalibur processing and instrument
    • control software
    • LCQUAN* 2.5 quantification package
    • Microsoft* Office XP software package
    • Microsoft Windows* XP operating system
    • High-performance PC with Intel* Pentium* microprocessor
    • High-resolution LCD color monitor

    Scan Functions

    • Full-scan mass spectra for sensitive analyses and rapid screening of unknown compounds
    • Selected Ion Monitoring (SIM) for selected ions for target compound analysis
    • Full-scan product ion spectra at sensitivities higher than any ion trap mass spectrometer
    • Selected Reaction Monitoring (SRM) for a traditional LC/MS/MS quantitative analytical experiment
    • MSn for multi-stage MS experiments to probe the structure of ions
    • ZoomScan,™ a high-resolution, full-range scan to resolve isotopic envelopes often used for charge state determination of peptides and ionization state determination of organometallics
    • Ultra ZoomScan for ultimate resolution
    • TurboScan*, an ultra-fast scan to improve signal-to-noise and sampling rate

    Exclusive Technologies

    • Pulsed Q Collision Induced Dissociation (PQD) enables trapping of low mass fragment ions
    • Electron Transfer Dissociation (ETD) enabled capability delivers peptide sequence information not available from conventional methods
    • High Resolution Isolation (HRI) allows for the separation of an isobaric interfering species down to 0.3 Da or for isolation of a thermally labile compound
    • Unique, proprietary Automatic Gain Control (AGC*) ensures that the ion trap is always filled with the optimum number of ions for any scan type
    • Dynamic Exclusion* allows acquisition of MS/MS and MSn spectra from lower intensity ion species
    • WideBand Activation* generates more structurally informative spectra
    • Normalized Collision Energy* compensates for the mass-dependent energy deposition characteristics of ion trap mass spectrometers in MS/MS experiments, providing reproducible data from instrument to instrument
    • Stepped Normalized Collision Energy allows for the variation of the collision energies in an experiment
    • Multistage Activation generates combination MS/MS spectra and MS3 spectra based on a user defined neutral loss

    Advanced Data Dependent Experiments

    • Data Dependent features trigger acquisition of MSn spectra only when a compound of interest is detected
    • Isotopic Data Dependent scanning software triggers MS/MS scanning only when a specified isotopic pattern is detected
    • Triple Play determines the charge state and MS/MS scan range of a multiply charged ion
    • Nth Order Triple Play allows the number of ions undergoing a Triple Play to be defined
    • Data Dependent Neutral Loss (NL) MS3 triggers MS3 scan only on the MS/MS product ions with the pre-defined neutral loss to identify and characterize metabolites and post-translational modifications
    • Ion Mapping* automatically generates a 3-dimensional MS/MS map, yielding product ion, precursor ion, and neutral loss information
    • Ion Mapping Browser Software displays data generated by Ion Mapping experiments
    • Data Dependent Ion Tree performs MSn experiments on up to 25 species
    • Data Dependent Zoom Map generates sequential MS/MS experiments using a ZoomScan for charge state determination prior to each MS/MS experiment
    • MSn Browser software displays data generated by Data Dependent Ion Tree and Ion Mapping experiments

    Optional Application-Specific Software

    • MetWorks* – automated metabolite identification using spectral trees
    • Mass Frontier* – spectral interpretation and classification software to identify unknowns
    • PEAKS – powerful, easy de novo sequencing
    • ProMass* Deconvolution – intact protein analysis
    • SIEVE* – automated label-free differential expression of proteins and peptides
    • ProSight PC* – top-down protein identification