Stanley Stevens
Stanley Stevens
Assistant Professor
Contact
Office: BSF 167
Phone: 813/974-8146
Email:
Education
Ph.D., University of Florida, 2001
Research
My research focuses on the development and application of novel proteomics-based approaches to understand how certain post-translational modifications (PTMs) such as phosphorylation and nitration influence protein and ultimately cell organelle function in various pathophysiological states. In particular, advanced biochemical and mass spectrometric techniques are utilized in my lab to determine changes in expression and PTMs of proteins derived from organelle structures of various cell and tissue types. Together with in vivo activity-based protein profiling, we are applying these methodologies to gain mechanistic insight into the complex cellular and biochemical changes in the brain and other target organ systems that occur after exposure to certain drugs of abuse. My group is currently engaged in efforts to
- Characterize activated signaling pathways in the drug reward circuitry of the brain during chronic opiate exposure with emphasis on the identification and site-specific quantitation of cyclin-dependent kinase 5 (CDK5)-mediated phosphorylation events. The role of CDK5 in other central nervous system-related disorders is also being investigated.
- Identify targets of inducible nitric oxide sythase (iNOS)-mediated mitochondrial protein nitration during alcohol-induced nitrosative stress in the liver.
Recent Publications
Stevens Jr., S. M.; Prokai-Tatrai, K.; Prokai, L.; “Factors that Contribute to the Misidentification of Tyrosine Nitration by Shotgun Proteomics”; Mol. Cell. Proteomics, in press (2008).
Zhang, J.; Planey, S. L.; Ceballos, C.; Stevens Jr., S. M.; Keay, S. K.; Zacharias, D. A.; “Identification of CKAP4/p63 as a Major Substrate of the Palmitoyl Acyl Transferase DHHC2, a Putative Tumor Suppressor, Using a Novel Proteomic Method”; Mol. Cell. Proteomics, 7, 1378-1388 (2008).
Stevens Jr., S. M.; Duncan, R. S.; Koulen, P.; Prokai, L.; “Proteomic Analysis of Mouse Brain Microsomes: Identification and Bioinformatic Characterization of Endoplasmic Reticulum Proteins in the Mammalian CNS”; J. Proteome Res., 7, 1046-1054 (2008).
Wasdo, S. C.; Barber, D. S.; Denslow, N. D.; Powers, K. W.; Palazuelos, M.; Stevens Jr., S. M.; Moudgil, B. M.; Roberts, S. M.; “Differential Binding of Serum Proteins to Nanoparticles”; Int. J. Nanotechnol., 5, 92-115 (2008).
Prokai, L; Frycak, P.; Stevens Jr., S. M.; Nguyen, V.; “Measurement of Acetylcholine in Rat Brain Microdialysates by LC–Isotope Dilution Tandem MS”; Chromatographia, 68, S101-S105 (2008).
Bhutia, S. K.; Mallick, S. K.; Stevens, S. M.; Prokai, L.; Vishwanatha, J. K.; Maiti, T. K.; “Induction of mitochondria-dependent apoptosis by Abrus agglutinin derived peptides in human cervical cancer cell”; Toxicol. In Vitro, 22, 344-351 (2008).
Stevens Jr., S. M.; Rauniyar, N.; Prokai, L; “Rapid Characterization of Covalent Modifications to Rat Brain Mitochondrial Proteins after ex vivo Exposure to 4-Hydroxy-2-Nonenal by Liquid Chromatography–Tandem Mass Spectrometry Using Data Dependent and Neutral Loss-Driven MS3 Acquisition”; J. Mass Spectrom., 42, 1599-1605 (2007).
Prokai, L.; Yan, L-J; Vera-Serrano, J. L.; Stevens Jr., S. M.; Forster, M. J.; “Mass Spectrometry-Based Identification of Mitochondrial Proteins Susceptible to Age-Related Oxidative Carbonylation in the Rat Brain”; J. Mass Spectrom., 42, 1583-1589 (2007).
Rauniyar, N.; Stevens Jr., S. M.; Prokai, L.; “Fourier-transform Ion-Cyclotron Resonance Mass Spectrometry of Covalent Adducts of Proteins and 4-Hydroxy-2-Nonenal, a Reactive End-Product of Lipid Peroxidation”; Anal. Bioanal. Chem., 389, 1421-1428 (2007).
Cardasis, H. L.; Stevens Jr., S. M.; McClung, S. H.; Kempler, K.; Powell, D. H.; Eyler, J. R.; Battelle, B. A.; “The Actin-Binding Interface of a Myosin III Is Phosphorylated in vivo in Response to Signals from a Circadian Clock”; Biochemistry, 46, 13907-13919 (2007).
Barber, D. S.; Stevens, S.; LoPachin, R. M.; “Proteomic Analysis of Rat Striatal Synaptosomes During Acrylamide Intoxication at a Low Dose-Rate”; Toxicol. Sci., 100, 156-167 (2007).
Perera O.; Green T. B.; Stevens Jr., S. M.; White S.; Becnel, J. J. Proteins Associated with Culex nigripalpus Nucleopolyhedrovirus (CuniNPV) Occluded Virions. J. Virol., 81, 4585-4590 (2007).
Kempler, K.; Tóth, J.; Yamashita, R.; Stevens Jr., S. M.; Cardasis, H.; Robinson, K.; Mapel, G.; Sellers, J.; Battelle, B. A. Loop 2 of Limulus myosin III is phosphorylated by protein kinase A and autophosphorylation. Biochemistry, 46, 4280-4293 (2007)