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Stanley  Stevens

Stanley Stevens

Stanley Stevens
Associate Professor, CMMB Research Director


Office: BSF 167
Phone: 813/974-8146
Lab: BSF119, 121


Personal Bio



Ph.D., University of Florida, 2001


The overproduction of reactive oxygen species (ROS) can lead to unfavorable reactions in the cell including direct oxidative modification of proteins as well as reaction of ROS with other cellular components such as lipids to form highly reactive carbonyl compounds. In addition to ROS, reactive nitrogen species such as peroxynitrite (ONOO-) can also disrupt normal cellular processes through potentially activity-altering protein modifications including the nitration of tyrosine residues. Mass spectrometry (MS)-based proteomics has become a powerful approach to identify and quantify these sites of oxidative protein modifications given the sensitivity and scan rate achievable by current MS instrumentation typically used for global-scale protein analysis. Our general research focuses on bioanalytical methods development in the field of proteomics with application to understanding the molecular mechanisms underlying cellular dysfunction due to oxidative/nitrosative stress. We are currently investigating the impact of ethanol-induced oxidative stress on epigenetic modifications that includes histone modifications and DNA methylation in various cell types.

Graduate Students

Brandi Cook, Joao Paulo Costa Pinho, Jennifer Guergues, Crystina Kriss-Manick, Daniel Martin, Richard Nguyen

Recent Journal Articles


Honors and Award






Recent Publications


(Since 2008)

  • Chaput, D.; Kirouac, L. H.; Bell-Temin, H.; Stevens, Jr., S. M.; Padmanabhan, J.; “Potential role of PCTAIRE-2, PCTAIRE-3 and P-Histone H4 in amyloid precursor protein-dependent Alzheimer pathology”; Oncotarget, 7, 8481-8497 (2016).
  • Martin, D. R.; Dutta, P.; Mahajan, S.; Varma, S.; Stevens, Jr., S. M.; “Structural and activity characterization of human PHPT1 after oxidative modification”; Nat. Sci. Rep., 6: 23658 (2016).
  • Zhang, P.*; Cochran-Culver, A.*; Stevens, Jr., S. M.; Liu, B.; “Characterization of a SILAC method for proteomic analysis of primary rat microglia”; Proteomics, 16, 1341-1346 (2016).
  • Narayan, M.; Kirouac, L.; Chaput, D.; Stevens, Jr., S. M.; Padmanabhan, J.; Jinwal, U. K.; “Identification of Novel Cdc37 Interacting Proteins and Pathways in Human Alzheimer's Disease Brain Tissue using Mass Spectrometry”; J. Data Mining Genomics Proteomics, 7(2): 1000193 (2016).
  • Bahia, P. K.; Parks, T. A.; Stanford, K. R.; Mitchell, D. A.; Varma, S.; Stevens, Jr., S. M.; Taylor-Clark, T. E.; “The exceptionally high reactivity of Cys 621 is critical for electrophilic activation of TRPA1”; J. Gen. Physiol., 147, 451-465 (2016).
  • Fontaine, S. N.; Sabbagh, J. J.; Martin, M.; Chaput, D.; Darling, A.; Trotter, J.; Zheng, D.; Stothert, A.; Nordhues, B.; Lussier, A.; Baker, J.; Shelton, L.; Blair, L.; Stevens, Jr., S. M.; Dickey, C. A.; “DnaJ/Hsc70 chaperone complexes control the extracellular release of neurodegenerative-associated proteins”; EMBO., 35, 1537-1549 (2016).
  • Athanason, M. G., Ratliff, W. A., Chaput, D., MarElia, C. B., Kuehl, M. N., Stevens, Jr., S. M., Burkhardt, B. R.; “Quantitative proteomic profiling reveals hepatic lipogenesis and liver X receptor activation in the PANDER transgenic model”; Mol. Cell. Endocrinol., in press (2016).
  • Mahajan, S.; Manetsch, R.; Merkler, D. J.; Stevens, Jr., S. M.;  “Synthesis and Evaluation of a Novel Adenosine-Ribose Probe for Global-Scale Profiling of Nucleoside-Binding Proteins”; PLoS One, 10, e0115644 (2015).
  • Krute, C. N.; Bell-Temin, H.; Miller, H. K.; Rivera, F. E.; Stevens, Jr., S. M.; Shaw, L. N.; “The Membrane Protein PrsS Parallels sS in Protecting Staphylococcus aureus Against Cell Wall-Targeting Antibiotics and DNA-Damaging Agents”; Microbiology, 161, 1136-1148 (2015).
  • Fontaine, S. N.; Rauch, J. N.; Stothert, A.; Assimon, V.; Cockman, M.; Chang, L; Sabbagh, J. J.; Nordhues, B. A.; Jinwal, U. K.; Stevens, Jr., S. M.; Zweckstetter, M.; Zuiderweg, E. R. P.; Gestwicki, J. E.; Dickey, C. A.; “Isoform Selective Genetic Inhibition of Constitutive Cytosolic Hsp70 Activity Promotes Client Tau Degradation Using an Altered Co-chaperone Complement”; J. Biol. Chem, 290, 13115-13127 (2015).
  • Barker, A.; Dombrosky, J.; Wolverton, S.; Venables, B.; Chaput, D.; Stevens, Jr., S. M.; “Validation of a non-targeted LC-MS approach for identifying ancient proteins: method development on bone to improve artifact residue analysis”; Ethnobiology Letters, 6, 162-174 (2015).
  • Copes, N.; Edwards, C.; Chaput, D; Saifee, M; Barjuca, I.; Nelson, D.; Paraggio, A.; Saad, P.; Lipps, D.; Stevens, Jr., S. M.; Bradshaw, P. C.; “Metabolome and proteome changes with aging in Caenorhabditis elegans”; Exp. Gerontol., 72, 67-84 (2015).
  • Liu, P.; Robbins, A.; Marzahn, M.; McClung, S.; Yowell, C.; Stevens, Jr., S. M.; Dame, J.; Dunn, B.; “Enzymatic characterization of recombinant food vacuole plasmepsin 4 from the rodent malaria parasite Plasmodium berghei”; PLoS One, 10, e0141758 (2015).
  • Bell-Temin, H.; Cochran-Culver, A.; Chaput, D.; Kuehl, M.; Carlson, C.; Bickford, P; Burkhardt, B.; Liu, B; Stevens, Jr., S. M.; " Novel molecular insights into classical and alternative activation states of microglia as revealed by SILAC-based proteomics"; Mol. Cell Proteomics, 14, 3173-3184 (2015).
  • Seeley, K. W.; Fertig, A. R.; Dufresne, C. P.; Pinho, J. C.; Stevens, Jr., S. M.; “Nitrotyrosine identification and relative quantitation using a stable isotope-labeled nitrated spike-in standard and high resolution Fourier transform MS and MS/MS analysis”; Special Issue: Fourier Transform Mass Spectrometry in Molecular Sciences, Int. J. Mol. Sci., 15, 6265-6285 (2014).
  • Yin, H.; Hu, M.; Liang, X.; Ajmo, J. M.; Li, X.; Stevens, Jr., S. M.; You, M.; “Genetic Ablation of Hepatic SIRT1 Disrupts Hepatic Lipin-1 Signaling and Results in Aggravated Alcoholic Steatohepatitis in Mice”; Gastroenterology, 146, 801-811 (2014).
  • Cooperman, C. E.; Dougan, G. D., Moak, S; Athanason, M.; Kuehl, M.; Bell-Temin, H.; Stevens Jr., S. M.; Burkhardt, B.; “PANDER transgenic mice display fasting hyperglycemia and insulin resistance”; J. Endocrinology, 220, 219-231 (2014). 
  • Morgan, J. K.; Vendura, K. W.; Stevens, Jr., S. M.; Riordan, J. T.; “Contribution of RcsB to the locus of enterocyte effacement (LEE) expression and adherence phenotype of virulent 2006 U.S. spinach outbreak Escherichia coli O157:H7 strain TW14359”; Microbiology, 159, 2342–2353 (2013).
  • Quintero, O. A.; Unrath, W. C.; Stevens Jr., S. M.; Manor, U.; Kachar, B.; Yengo, C. M.; “Myosin 3A kinase activity is regulated by phosphorylation of the kinase domain activation loop”; J. Biol. Chem., 288, 37126-37137 (2013).
  • Bell-Temin, H.; Zhang, P.; Chaput, D.; King, M. A.; You, M.; Liu, B.; Stevens, Jr., S. M.; “Quantitative proteomic characterization of ethanol-responsive pathways in rat microglial cells”; J. Proteome Res., 12, 2067-2077 (2013).
  • Xu, G.; Stevens, Jr., S. M.; Moore, B. D.; McClung, S.; Borchelt, D. R. “Secondary misfolding of soluble cytosolic proteins in a transgenic mouse model of Alzheimer-amyloidosis”; Hum. Mol. Genet., 22, 2765-2774 (2013).
  • Kolar, S. L.; Rivera, F. E.; Mootz, J. M.; Davenport, J. E.; Stevens, Jr., S. M.;  Horswill, A. R.; Shaw, L. N.; "Extracellular Proteases are Key Mediators of S. aureus Virulence via the Global Modulation of Virulence Determinant Stability"; Microbiology Open, 2, 18-34 (2013).
  • Seeley, K. W.; Stevens, Jr., S. M.; “Investigation of local primary structure effects on peroxynitrite-mediated tyrosine nitration using targeted mass spectrometry”; J. Proteomics, 75, 1691-1700 (2012).
  • Bell-Temin, H.; Barber, D. S.; Liu, B.; Stevens, Jr., S. M.; “Proteomic Analysis of Rat Microglia Establishes a High-Confidence Reference Dataset of Over 3,000 Proteins”; Proteomics , 12, 246-250 (2012). 
  • Chaput, D.; Kirouac, L. H.; Bell-Temin, H.; Stevens, Jr., S. M.* Padmanabhan, J.*; “SILAC-based proteomic analysis to investigate the impact of amyloid precursor protein expression in neuronal-like B103 cells”; Neuroproteomics Special Issue, Electrophoresis, 33, 3728-3737 (2012).
  • Xu, G.; Stevens, Jr., S. M.; Kobiessy, F.; Brown, H.; McClung, S.; Gold, M. S.; Borchelt, D. R.; "Identification of proteins sensitive to thermal denaturation in human neuroblastoma and glioma cell lines"; PLoS One, 7, e49021 (2012).
  • Rivera, F. E.; Miller, H. K.; Kolar, S. L.; Stevens, Jr., S. M.; Shaw, L. N.; “The Impact of CodY on Virulence Determinant Production in Community-Associated Methicillin Resistant Staphylococcus aureus” Proteomics, 12, 246-250 (2012).
  • Liu, B.; Barber, D. S.; Stevens Jr., S. M.; “Stable Isotope Labeling with Amino Acids in Cell Culture (SILAC)-based Proteomic Analysis of Ethanol-Induced Protein Expression Profiles in Microglia”; Methods Mol. Biol. 829, 551-565 (2012).
  • Maldonado-Devincci, A. M.; Stevens Jr., S. M.; Kirstein, C. L.; “Investigation of Age-Specific Behavioral and Proteomic Changes in an Animal Model of Chronic Ethanol Exposure”; Methods Mol. Biol., 829, 471-485 (2012).
  • Barker, A.; Venables, B.; Stevens Jr., S. M.; Seeley, K. W.; Wang, P.; Wolverton, S.; “An optimized approach for protein-residue extraction from experimental cooking pottery”; J. Archaeol. Method Th., 19, 407-439(2012).
  • Dalal, J.S.; Stevens, Jr., S. M.; Alvarez, S.; Munoz, N.; Kempler, K.E.; Dose, A.C.; Burnside, B.; Battelle, B-A. “Mouse Class III myosins: Kinase activity and phosphorylation sites”; J. Neurochem., 119, 772-784 (2011).
  • Guingab-Cagmat, J. D.; Stevens Jr., S. M.; Ratliff, M. V.; Zhang, Z.; Gold, M. S.; Wang, K. W.; Kobeissy, F. H.; “Identification of Tyrosine Nitration in UCHL1 and GAPDH”; Electrophoresis,32,1692–1705 (2011).
  • Stevens Jr., S. M.; Wolverton, S.; Venables, B.; Barker, A.; Seeley, K. W.; Adhikari, P.; “Evaluation of microwave-assisted enzymatic digestion and tandem mass spectrometry for the identification of protein residues from an inorganic solid matrix: implications in archaeological research”; Anal. Bioanal. Chem., 396, 1491-1499 (2010).
  • Westerheide, S. D.; Anckar, J.; Stevens Jr., S. M.; Sistonen, L.; Morimoto, R. I.; “Stress-Inducible Regulation of Heat Shock Factor 1 by the Deacetylase SIRT1”; Science, 323, 1063-1066 (2009).
  • Rauniyar, N.; Stevens Jr., S. M.; Prokai-Tatrai, K.; Prokai, L.; “Characterization of 4-Hydroxy-2-Nonenal-Modified Peptides by Liquid Chromatography Tandem Mass Spectrometry Using Data-Dependent Acquisition: Neutral Loss-Driven MS3 versus Neutral Loss-Driven Electron Capture Dissociation”; Anal. Chem., 81, 782-789 (2009).
  • Liu, P.; Marzahn, M. R.; Robbins, A. H.; Gutiérrez-de-Terán, H.; Rodríguez, D.; McClung, S. H.; Stevens Jr., S. M.; Yowell, C. A.; Dame, J. B.; McKenna, R.; Dunn, B. M.; “Recombinant plasmepsin 1 from the human malaria parasite plasmodium falciparum: enzymatic characterization, active site inhibitor design, and structural analysis”;Biochemistry, 48, 4086-4099 (2009). 
  • Prokai, L.; Stevens, S. M.; Rauniyar, N.; Nguyen, V.; “Rapid Label-Free Identification of Estrogen-Induced Differential Protein Expression In Vivo from Mouse Brain and Uterine Tissue”; J. Proteome Res., 8, 3862-3871 (2009). 
  • Stevens Jr., S. M.; Prokai-Tatrai, K.; Prokai, L.; “Factors that Contribute to the Misidentification of Tyrosine Nitration by Shotgun Proteomics”; Mol. Cell. Proteomics , 7, 2442-2451 (2008).
  • Zhang, J.; Planey, S. L.; Ceballos, C.; Stevens Jr., S. M.; Keay, S. K.; Zacharias, D. A.; “Identification of CKAP4/p63 as a Major Substrate of the Palmitoyl Acyl Transferase DHHC2, a Putative Tumor Suppressor, Using a Novel Proteomic Method”; Mol. Cell. Proteomics, 7, 1378-1388 (2008).
  • Stevens Jr., S. M.; Duncan, R. S.; Koulen, P.; Prokai, L.; “Proteomic Analysis of Mouse Brain Microsomes: Identification and Bioinformatic Characterization of Endoplasmic Reticulum Proteins in the Mammalian CNS”; J.Proteome Res., 7, 1046-1054 (2008).
  • Wasdo, S. C.; Barber, D. S.; Denslow, N. D.; Powers, K. W.; Palazuelos, M.; Stevens Jr., S. M.; Moudgil, B. M.; Roberts, S. M.; “Differential Binding of Serum Proteins to Nanoparticles”; Int. J. Nanotechnol., 5, 92-115 (2008).
  • Prokai, L; Frycak, P.; Stevens Jr., S. M.; Nguyen, V.; “Measurement of Acetylcholine in Rat Brain Microdialysates by LC–Isotope Dilution Tandem MS”; Chromatographia, 68, S101-S105 (2008).
  • Bhutia, S. K.; Mallick, S. K.; Stevens, S. M.; Prokai, L.; Vishwanatha, J. K.; Maiti, T. K.; “Induction of mitochondria-dependent apoptosis by Abrus agglutinin derived peptides in human cervical cancer cell”; Toxicol. In Vitro, 22, 344-351 (2008).