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James  Riordan

James Riordan

James Riordan
Instructor II, CMMB Director of Undergraduate Studies

Contact

Office: ISA 6206
Phone: 813/974-7457
Lab: ISA 6014
Fax: 813/905-9919
Email:

Links

Education

Ph.D. Molecular Biology, New Mexico State University, 2006.

Professional Experience

Assistant Professor, University of South Florida, 2009-Present

Research

Regulation of virulence and stress fitness in pathogenic E. coli:

Alternative sigma factor N:
Sigma N (encoded by rpoN) is an alternative sigma subunit of the RNA polymerase machinery that confers specificity to transcription initiation under specific growth conditions. We have determined that the deletion of rpoN in E. coli modulate the expression of gene clusters involved in resistance to acid (important for low infectious dose and for transmission), as well as those encoded on a pathogenicity island (the LEE) important for gut colonization (Riordan et al. 2009, Microbiology 156(Pt. 3):719. PubMed); (Mitra et al. 2012, PLoS One. 7(9): e46288 (PMID 23029465) PubMed. Current studies examine the stimuli and mechanisms by which sigma N is activated to control acid resistance and the LEE, and the significance of this control to disease progression.

Rcs phosphorelay:
Rcs is a multi-component phosphorelay system in E. coli which transmits various environmental signals to the transcriptional machinery. We have determined that the Rcs system is required for activation of the locus of enterocyte effacement (LEE) in response to physiologic bicarbonate in the intestine (Morgan et al. 2013, Microbiology 159:2342. PubMed). Adding to this, a reciprocating pathway has been identified by our group in which Rcs and the LEE-encoded regulator GrlA cooperatively repress motility. These observations have led us to hypothesize that Rcs phosphorelay coordinates LEE activation and adherence with LEE-directed repression of motility and dispersion in response to gut stimuli.

***The overarching goal of our research is to understand how pathogenic E. coli cause intestinal infections in an effort to develop or modify strategies for disease prevention and control.

Current Courses

RefCourseSecCourse TitleCRDayTimeLocation
93074MCB 3020001General Microbiology
3MW

8:00am-9:15am

ISA 1051

93075MCB 3020002General Microbiology
SECTION FOR LECTURE RETAKE ONLY
3MW

8:00am-9:15am

ISA 1051

93076MCB 4115001Determinative Bacteriology
3TR

11:00am-12:15pm

CHE 101

93288MCB 4115L002Determinative Bacteriology Lab
2MW

11:50am-1:40pm

ISA 3026

93289MCB 4115L003Determinative Bacteriology Lab
2TR

8:35am-10:25am

ISA 3026

93290MCB 4115L004Determinative Bacteriology Lab
2TR

12:30pm-2:20pm

ISA 3026

93651MCB 4503002Virology
This class will be team taught by Drs. Riordan and Burkhardt.
3MW

11:00am-12:15pm

CHE 217

93784BSC 6932084Microbial Processes
For INTO students only.
3MW

8:00am-9:15am

ISA 1051