Daniel Lim
Daniel Lim
Distinguished University Professor
Contact
Office: IDRB ISA 404 6207
Phone: 813/974-1618
Email:
Links
Education
B.A., Rice University, 1970.
Ph.D., Texas A&M University, 1973.
Postdoctoral Fellow, Baylor College of Medicine, 1973-1976.
Research
Pathogenic/Molecular/Applied Microbiology
Virulence mechanisms of pathogenic bacteria; rapid identification of pathogens; sample processing; biosensors; public health relevance of bacterial pathogens
The primary focus of our research is the characterization of virulence mechanisms in pathogenic bacteria and development of innovative technology to rapidly detect and identify microbial pathogens in food, water, air, surfaces, and infectious diseases.
Conventional procedures to detect and identify pathogens from complex matrices can take days to perform and may provide inconclusive information. Our research focuses on development of sample processing procedures and assays for rapid, accurate, and reproducible detection of pathogenic microorganisms. We have developed rapid processing methods and concentration protocols for complex matrix samples such as ground beef, apple juice, produce, potable water, recreational water, human blood, and saliva, enabling such samples to be directly tested by biosensors and other detection platforms for target analytes. Assays developed by our laboratory for agents such as Bacillus anthracis, E. coli O157:H7, Vibrio cholerae, Vaccinia virus, and ricin have been optimized for reproducibility, sensitivity, and specificity, and tested on various detection platforms. Our assay protocols include orthogonal testing to improve detection specificity and automated concentration procedures to improve detection sensitivity. We also have studied problems associated with target analyte capture efficiency and have developed methods to improve capture efficiency and assay sensitivity using antibodies and other capture molecules. This research, which is performed in close collaboration with federal and local public health agencies, food processors, and water utilities, is supported by the Department of Defense, NIH, NSF, EPA, and other agencies, and can significantly improve public health through rapid detection of microbial pathogens and reduction of disease morbidity and mortality.
Honors and Award
Homeland Security Award in the Biological, Radiological, Nuclear Field.
For more information please visit this site.
Recent Publications
DeMarco, D.R. and D.V. Lim. 2001. Direct detection of Escherichia coli 0157:H7 in unpasteurized apple juice with an evanescent wave biosensor. J. Rapid Meth. Automat. Microbiol. 9:241-257.
Lim, D.V. 2001. Rapid biosensor detection of foodborne microbial pathogens. Microbiol. Meth. Forum Newslett. 18:13-17.
Tims, T.B., S.S. Dickey, D.R. DeMarco, and D.V. Lim. 2001. Detection of low levels of Listeria monocytogenes within 20 hours using an evanescent wave biosensor. Am. Clin. Labor. 20:28-29.
DeMarco, D.R. and D.V. Lim. 2002. Detection of Escherichia coli 0157:H7 in 10- and 25-gram ground beef samples using an evanescent wave biosensor with silica and polystyrene waveguides. J. Food Protect. 65: 596-602.
Kramer, M.F., T.B. Tims, and D.V. Lim. 2002. Recovery of Escherichia coli O157:H7 from optical waveguides used for rapid biosensor detection. J. Rapid Meth. Automat. Microbiol. 10:93-106.
Turos, E., T.E. Long, M.I. Konaklieva, C. Coates, J-Y. Shim, S. Dickey, D.V. Lim, and A. Cannons. 2002. N-Thiolated ß-lactams: Novel antimicrobial agents for methicillin-resistant Staphylococcus aureus. Bioorg. Med. Chem. Lett. 12:2229-2231.
Coates, C., T.E. Long, E. Turos, S. Dickey, and D.V. Lim. 2003. N-Thiolated ß-lactam antibacterials:Defining the role of unsaturation in the C4 side chain. Bioorg. Med. Chem. 11:193-196.
Lim, Daniel V. 2003. Real time/near real time detection of bioterrorism agents. Biodetection Technologies: Identification Innovations and Strategies, pp. 117-136. Knowledge Press, Brookline, MA.
Long, T.E., E. Turos, M.I. Konaklieva, A. L. Blum, A. Amry, E.A. Baker, L.S. Suwandi, M.D. McCain, M.F. Rahman, S. Dickey, and D.V. Lim. 2003. Effect of aryl ring fluorination on the antibacterial properties of C4 aryl-substituted N-methylthio ß-lactams. Bioorg. Med. Chem. 11:1859-1863.
Lim, D.V. 2003. Detection of microorganisms and toxins with evanescent wave, fiber optic biosensors. Proc. IEEE. 91: 902-907.
Tims, T.B. and D.V. Lim. 2003. Confirmation of viable E. coli O157:H7 by enrichment and PCR after rapid biosensor detection. J. Microbiol. Meth. 55:141-147.
Kramer, M.F. and D.V. Lim. 2004. A rapid and automated fiber optic-based biosensor assay for the detection of Salmonella in spent irrigation water used in the sprouting of sprout seed. J. Food Protect. 67:46-52.
Harwood, V.J., N.C. Delahoya, R.M. Ulrich, M.F. Kramer, J.E. Whitlock, J.R. Garey, and D.V. Lim. 2004. Molecular confirmation of Enterococcus faecalis and E. faecium from clinical, fecal and environmental sources. Letts. Appl. Microbiol. 38:476-482.
Donaldson, K.A., M.F. Kramer, and D.V. Lim. 2004. A rapid detection method for Vaccinia virus, the surrogate for smallpox virus. Biosens. Bioelectron. 20:322-327.
Tims, T.B. and D.V. Lim. 2004. Rapid detection of Bacillus anthracis spores directly from powders with an evanescent wave fiber-optic biosensor. J. Microbiol. Meth. 59:127-130.
Nyquist-Battie, C., L.E. Frank, D. Lund, and D.V. Lim. 2004. Optimization of enzyme-linked immunosorbent assay detection of Escherichia coli O157:H7 in apple juice. J. Food Protect. 67:2756-2759.
Liam, W., S.A. Litherland, H. Badrane, W. Tang, D. Wu, H.V. Baker, P.A. Gulig, D.V. Lim, and S. Jin. 2004. Ultra-sensitive detection of biomolecules with fluorescent dye-doped nanoparticles. Anal. Biochem. 334:135-144.
Simpson, J.M. and D.V. Lim. Rapid PCR Confirmation of E. coli O157:H7 after evanescent wave fiber optic biosensor detection. 2005. Biosens. Bioelectron. 21:881-887
Nyquist-Battie, C., L. Mathias, L.E. Frank, D. Lund , and D.V. Lim. 2005. Antibody-based detection of acid-shocked, acid-adapted, and apple juice-incubated Escherichia coli O157:H7. J. Immunoassay Immunochem. 26:259-271.
Turos, E., C. Coates, J-Y Shim, Y. Wang, J.M. Leslie, T.E. Long, G.S.K. Reddy, A. Ortiz, M. Culbreath, S. Dickey, D.V. Lim, E. Alonso, and J. Gonzalez. 2005. N -Methylthio ß-lactam antibacterials: effects of the C 3 /C 4 ring substituents on anti-MRSA a ctivity. Bioorg. Med. Chem. 13:6289-2308.
Lim, D.V., J.M. Simpson, E.A. Kearns, and M.F. Kramer. 2005. Monitoring for agents of bioterrorism/biowarfare. Clin. Microbiol. Rev. 18:583-607.
Turos, E., R.K. Mishra, K.D. Revell, C.M. Coates, S. Dickey, and D.V. Lim. 2006. N -Thiolated 2-oxazolidinones: a new family of antibacterial agents for methicillin-resistant Staphylococcus aureus (MRSA) and Bacillus anthracis. Bioorg. Med. Chem. Lett. 16:2081-2083.
Turos, E., T.E. Long, B. Heldreth, J.M. Leslie, S.K.R. Guntireddygari, Y. Wang, C. Coates, M. Konaklieva, S. Dickey, D.V Lim, E. Alonso, and J. Gonzalez. 2006. N- Thiolated ß -lactams: a new family of anti- Bacillus agents. Bioorg. Med. Chem. Lett. 16:2084-2090.
Heldreth, B., T.E. Long, S. Jang, G.S.K. Reddy, E. Turos, S. Dickey, and D. V. Lim. 2006. N-Thiolated ß-lactam antibacterials: effects of the N-organothio substituent on anti-MRSA activity. Bioorg. Med. Chem. 14:3775-3784.
Turos, E., J-Y. Shim, Y. Wang, K. Greenhalgh, G.S.K. Reddy, S. Dickey, and D.V. Lim. 2007. Antibiotic-conjugated polyacrylate nanoparticles: new opportunities for development of anti-MRSA agents. Bioorg. Med. Chem. Lett. 17:53-56.
Turos, E., S.K. Reddy, K. Greenhalgh, S.A. Abeylath, S. Dickey, and D.V. Lim. 2007. Penicillin-bound polyacrylate nanoparticles: restoring the activity of beta-lactam antibiotics against MRSA. Bioorg. Med. Chem. Lett. 17:3468-3472.
Nyquist-Battie, C., L. Freeman, J.P.S. Soberano, D. Lund, E. Kearns, and D.V. Lim. 2007. Effects of chlorine injury, starvation and Colitag enrichment on sandwich enzyme-linked immunosorbent assay (sELISA) detection of Escherichia coli O157:H7. J. Immunoassay Immunochem. 28:371-383.
Kramer, M.F., G. Vesey, N.L Look, B.R. Herbert, J.M. Simpson and D.V. Lim. 2007. Development of a Cryptosporidium oocyst assay using an automated fiber optic-based biosensor. J. Biol. Eng. 1:3.
Simpson-Stroot, J.M., E.A. Kearns, P.G. Stroot, S. Magaña, and D.V. Lim. 2008. Monitoring biosensor capture efficiencies: development of a model using GFP-expressing Escherichia coli O157:H7. J. Microbiol. Meth. 72:29-37.
Abeylath, S.C., E. Turos, S. Dickey, and D.V. Lim. 2008. Glyconanobiotics: Novel carbohydrated nanoparticle antibiotics for MRSA and Bacillus anthracis. Bioorg. Med. Chem. 16:2412-2418.
Turos, E., K.D. Revell, P. Ramaraju, D.A. Gergeres, K. Greenhalgh, A. Young, N. Sathyanarayan, S. Dickey, D.V. Lim, M.M. Alhamadsheh, K. Reynolds. 2008. Unsymmetric aryl-alkyl disulfide growth inhibitors of methicillin-resistant Staphylococcus aureus and Bacillus anthracis. Bioorg. Med. Chem. 16:6501-6508.
Brown, D.R., L.D. Wednland, G.J. Ortiz, M.F. Kramer, D.V. Lim, M.B. Brown, and P.A. Klein. 2008. Evanescent-wave biosensor for field serodiagnosis of tortoise mycoplasmosis. Vet. Immunol. Immunopathol. 124:322-331.
Kearns, E.A., S. Magana, and D.V. Lim. 2008. Automated concentration and recovery of microorganisms from drinking water using dead-end ultrafiltration. J. Appl. Microbiol. 105:432-442.
Leskinen, S.D. and D.V. Lim. 2008. Rapid ultrafiltration concentration and biosensor detection of enterococci from large volumes of Florida recreational water. Appl. Environ. Microbiol. 74:4792-4798.
Nyquist-Battie, C., L. Freeman, K. Leckband, S. Martinez, A. Ansley, D. Lund, and D.V. Lim. 2008. Antibody-based detection of Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium grown in low-shear modeled microgravity. Micrograv. Sci. Technol. 20:23-28.
O’Driscoll, M., K. Greenhalgh, A. Young, E. Turos, S. Dickey, and D.V. Lim. 2008. Studies on the antifungal properties of N-thiolated beta-lactams. Bioorg Med. Chem. 16:7832-7837.
Denton, K.A., M.F. Kramer, and D.V. Lim. 2009. Rapid detection of Mycobacterium tuberculosis in lung tissue using a fiber optic biosensor. J. Rapid Meth. Automat. Microbiol. 17:17-31.
Leskinen, S.D., S.M. Schlemmer, E.A. Kearns and D.V. Lim. 2009. Detection of E. coli O157:H7 in complex matrices under varying flow parameters with a robotic, fluorometric assay system. Proc. SPIE 7167:71670J-1 – 71670J-10.
Lim, D.V., E.A. Kearns, S.D. Leskinen, S. Magaña, J. M. Stroot, D.M. Hunter and S. Schlemmer. Sample preparation and assay refinements for pathogen detection platforms. 2009. Proc. SPIE. 7167:71670O-1 – 71670O-10.
Leskinen, S.D.and D.V. Lim. 2009. Rapid dead-end ultrafiltration concentration and biosensor detection of enterococci from beach waters of Southern California. J. Water and Hlth. 7:674-684.
Garay-Jimenez, J.C., D. Gergeres, A. Young, S. Dickey, D.V. Lim, and E. Turos. 2009. Physical properties and biological activity of poly (butyl acrylate–styrene) nanoparticle emulsions prepared with conventional and polymerizable surfactants. Nanomedicine: Nanotechnology, Biology and Medicine. 5:443-451.
Hunter, D.M. and D.V. Lim. 2010. Rapid detection and identification of bacterial pathogens using an ATP bioluminescence immunoassay. J. Food Protect. In press.