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CMMB Division
Department of Biology
Joyce  Stroot

Joyce Stroot

Joyce Stroot
Research Associate, Lim Lab, Microbiology and Molecular Biology

Contact

Office: IDRB 404
Phone: 813/974-1316
Email:

Bio

I am primarily interested in using molecular and microbiological tools to solve environmental problems. My research has covered a wide range of topics, some of which include: bioremediation of crude oil spills; probiotic Lactobacilli and their effects on the gastrointestinal ecology of weaning pigs; bioaugmentation of industrial waste streams; and fecal source tracking methodologies. My current interests are focused on fecal source tracking to improve water quality and effectively remediate chronic contamination issues. Ongoing research includes development of fiber-optic biosensor assays for use in field operations that target specific fecal bacteria and can identify sources of contamination in regard to host types (i.e. human vs. animal).

Education

Ph.D. - University of Illinois at Urbana-Champaign, 2001

Research

Microbiology and Molecular Biology.

Recent Publications

Simpson, J. M., V. J. McCracken, B. A. White, H. R. Gaskins, and R. I. Mackie. 1999. Optimization of Denaturant Gradient Gel Electrophoresis for the Analysis of the Porcine Gastrointestinal Microbiota. J. Microbiol. Methods. 36:167-179.

Anderson, D. B., V. J. McCracken, R. I. Aminov, J. M. Simpson, R. I. Mackie, M. W. Verstegen, and H. R. Gaskins. 1999. Gut Microbiology and Growth-Promoting Antibiotics in Swine. Pig News and Information. 20:115N-122N.

Simpson, J. M., V. J. McCracken, H. R. Gaskins, and R. I. Mackie. 2000. Denaturant Gradient Gel Electrophoresis Analysis of 16S Ribosomal DNA Amplicons to Monitor Changes in Fecal Bacterial Populations of Weaning Pigs after Introduction of Lactobacillus reuteri Strain MM53. Appl. Environ. Microbiol. 66:4705-4714

McCracken, V. J., J. M. Simpson, McCracken, B.A., R. I. Mackie, and H. R. Gaskins. 2001. Dietary and Antibiotic-Induced Alteration of the Mouse Intestinal Microbiota. J. Nutrition. 131:1862-1870.

Simpson, J. M., B. Martineau, W. E. Jones, J. M. Ballam, and R. I. Mackie. 2002. Characterization of Fecal Bacterial Populations in Canines: Effects of Age, Breed and Dietary Fiber. Microbial Ecol. 44:186-197.

Simpson, J. M., S. A. Kocherginskaya, R. I. Aminov, L. T. Skerlos, T. M. Bradley, R. I. Mackie, and B. A. White. 2002. Comparative Microbial Diversity in the Gastrointestinal Tracts of Food Animal Species. Integ. Comp. Biol. 42:327-331. (formerly American Zoologist)

Simpson, J. M., J. W. Santo Domingo, and D. J. Reasoner. 2002. Microbial Source Tracking - State of Science. Environ. Sci. & Tech. 36:5279-5388.

Santo Domingo, J.W., M. C. Meckes, J. M. Simpson, B. Sloss, and D. J. Reasoner. 2003. Molecular Characterization of Bacteria Inhabiting a Water Distribution System Simulator. Water Sci. Tech. 47:149-154.

Simpson, J. M., J. W. Santo Domingo, and D. J. Reasoner. 2004. Bacterial Diversity in a Sub-Watershed Stream Affected by Equine Fecal Contamination. FEMS Microbiol. Ecol. 47:65-75.

Dick, L. K., A. E. Bernhard, T. J. Brodeur, J. W. Santo Domingo, J. M. Simpson , S. P. Walters,and K. G. Field. 2005. Host Distributions of Uncultivated Fecal Bacteroidales Reveal Genetic Markers for Fecal Source Identification. Appl. Environ. Microbiol. 71:3184-3191.

Simpson, J. M. and D. V. Lim. 2005. Rapid PCR Confirmation of E. coli O157:H7 after Evanescent Wave Fiber Optic Biosensor Detection. Biosensor. Bioelect. 21:881-887.

Lim, D. V., J. M. Simpson , E. A. Kearns, and M. F. Kramer. 2005. Current and Developing Technologies for Monitoring Agents of Bioterrorism and Biowarfare. Clin. Microbiol. Rev. 18:583-607.

Simpson, J. M. , P. G. Stroot, S. Gelman, I. Beydilli, S. Dudley and D. B. Oerther. 2006. 16S Ribosomal RNA Tools Identify an Unexpected Predominance of Paenibacillus- like Bacteria in an Industrial Activated Sludge System Suffering from Poor Biosolids Separation. Water Environ. Res. 78:864-871.

Simpson, J. M. 2007. Monitoring Bioterrorism / Biowarfare Agents. In McGraw-Hill 2007 Yearbook of Science & Technology. (in press).