Patrick Bradshaw
Patrick Bradshaw
Assistant Professor
Contact
Office: BSF 166
Phone: 813/974-7103
Email:
Education
Ph.D. Biochemistry, Ohio State University, 2001.
Research
My research focuses on the study of mitochondrial dysfunction with age. Mitochondria are the powerhouses of the cell. Mitochondrial dysfunction is hypothesized to be a cause of aging and diseases associated with advanced age including heart disease, diabetes, and neurodegeneration. Protecting mitochondrial function is therefore a key strategy in disease prevention. My laboratory employs a multidisciplinary approach that employs traditional biochemical characterization of mitochondria, lifespan studies with transgenic mice, computational methods, and genomic and proteomic approaches. My group is working toward the following specific aims:
1) Determining the mechanisms that alter the expression of the master mitochondrial transcriptional regulator PGC-1a with age.
2) Discovering ways to prevent the increase in mitochondrial reactive oxygen species production and mitochondrial DNA deletion mutations that occur with age.
3) Using proteomics to identify changes in mitochondrial proteins that occur with age and with the anti-aging treatment of caloric restriction.
Current Courses
Recent Publications
Kujoth, G.C., Bradshaw, P.C., Haroon, S., and Prolla, T.A. (2007) The role of mitochondrial DNA mutations in mammalian aging. PLOS Genet. 3(2):e24.
Bradshaw, P.C., and Pfeiffer, D.R. (2006) Release of Ca2+ and Mg2+ from yeast mitochondria is stimulated by increased ionic strength. BMC Biochem. 7:4.
Bradshaw, P.C., and Pfeiffer, D.R. (2006) Loss of NAD(H) from swollen yeast mitochondria.
BMC Biochem. 7:3.
Bradshaw, P.C., and Samuels, D.C. (2005) A computational model of mitochondrial
deoxynucleotide metabolism and DNA replication. Am. J. Physiol.Cell Physiol. 288,
C989-C1003.
Bradshaw, P.C., Li, J., and Samuels, D.C. (2005) A computational model of mitochondrial AZT
metabolism. Biochem. J. 392, 363-373.
Bradshaw P.C., Rathi A, Samuels D.C. (2005) Mitochondrial-encoded membrane protein
transcripts are pyrimidine-rich while soluble protein transcripts and ribosomal RNA are
purine-rich. BMC Genomics. Sep 26;6:136.
Jung, D.W., Bradshaw, P.C., Litsky, M., and Pfeiffer, DR (2004) Ca2+ transport in mitochondria
from yeast expressing recombinant aequorin. Anal. Biochem. 324, 258-268.
Bradshaw, P.C., Jung, D.W., and Pfeiffer D.R. (2001) Free fatty acids stimulate a vigorous
Ca2+: 2 H+ exchange in yeast mitochondria. J. Biol. Chem. 276, 40502-40509.
Mannella C.A.., Pfeiffer D.R., Bradshaw P.C., Moraru I.I., Slepchenko B., Loew L.M., Hsieh
C.E., Buttle K., Marko M. (2001) Topology of the mitochondrial inner membrane: dynamics and bioenergetic implications. IUBMB Life 52, 93-100.
Jung, D.W., Bradshaw, P.C., and Pfeiffer, D.R. (1997) Properties of a cyclosporin- insensitive
permeability transition pore in yeast mitochondria. J. Biol. Chem. 272, 21104-21112.