Daniel Lim
Daniel Lim
Distinguished University Professor
Contact
Office: IDRB 404
Phone: 813/974-1618
Email:
Links
Education
Ph.D., Texas A&M University, 1973.
Postdoctoral Fellow at Baylor College of Medicine.
Research
Pathogenic/Molecular/Applied Microbiology
Virulence mechanisms of pathogenic bacteria; rapid identification of pathogens; fiber optic and array biosensors; public health relevance of bacterial pathogens
The primary focus of our research is the characterization of virulence mechanisms in pathogenic bacteria and development of innovative technology to rapidly detect and identify bacterial pathogens in food, water, air, surfaces, and infectious diseases.
Current research involves the development of fiber optic and array biosensor assays for the real time/near real time detection of pathogenic microorganisms. Biosensors utilize fiber optic or planar waveguides to capture and detect target analytes. Detection antibodies, receptor molecules, and/or nucleic acid probes immobilized on the waveguide selectively bind specific target analytes. By attaching a fluorescent-labeled antibody to the analyte antigen in a "sandwich" assay, the fluorophore can be excited by a laser to generate a detectable signal. This innovative system has been used to successfully detect pathogens such as E. coli O157:H7 directly (without enrichment) from ground beef, apple juice, and raw sewage at low levels. Other microbial pathogens, including Salmonella Typhimurium, Bacillus anthracis, Vibrio cholerae, Vaccinia virus, and Cryptosporidium, have been successfully detected with the biosensor. This research, which is performed in close collaboration with federal and local public health agencies, food processors, and water utilities, is funded by the Department of Defense, NIH, EPA, and other agencies, and can significantly improve public health through rapid detection of microbial pathogens and reduction of disease morbidity and mortality.
Current Courses
Honors and Award
Homeland Security Award in the Biological, Radiological, Nuclear Field.
For more information please visit this site.
Recent Publications
DeMarco, D.R. and D.V. Lim. 2001. Direct detection of Escherichia coli 0157:H7 in unpasteurized apple juice with an evanescent wave biosensor. J. Rapid Meth. Automat. Microbiol. 9:241-257.
Lim, D.V. 2001. Rapid biosensor detection of foodborne microbial pathogens. Microbiol. Meth. Forum Newslett. 18:13-17.
Tims, T.B., S.S. Dickey, D.R. DeMarco, and D.V. Lim. 2001. Detection of low levels of Listeria monocytogenes within 20 hours using an evanescent wave biosensor. Am. Clin. Labor. 20:28-29.
DeMarco, D.R. and D.V. Lim. 2002. Detection of Escherichia coli 0157:H7 in 10- and 25-gram ground beef samples using an evanescent wave biosensor with silica and polystyrene waveguides. J. Food Protect. 65: 596-602.
Kramer, M.F., T.B. Tims, and D.V. Lim. 2002. Recovery of Escherichia coli O157:H7 from optical waveguides used for rapid biosensor detection. J.Rapid Meth. Automat. Microbiol. 10:93-106.
Turos, E., T.E. Long, M.I. Konaklieva, C. Coates, J-Y. Shim, S. Dickey, D.V. Lim, and A. Cannons. 2002. N-Thiolated ß-lactams: Novel antimicrobial agents for methicillin-resistant Staphylococcus aureus. Bioorg. Med. Chem. Letts. 12:2229-2231.
Lim, D.V. 2003. Microbiology, Third Edition. Kendall/Hunt, Dubuque. IA. 800 pages.
Coates, C., T.E. Long, E. Turos, S. Dickey, and D.V. Lim. 2003. N-Thiolated ß-lactam antibacterials:Defining the role of unsaturation in the C4 side chain. Bioorg. Med. Chem. 11:193-196.
Lim, Daniel V. 2003. Real time/near real time detection of bioterrorism agents. Biodetection Technologies: Identification Innovations and Strategies, pp. 117-136. Knowledge Press, Brookline, MA.
Long, T.E., E. Turos, M.I. Konaklieva, A. L. Blum, A. Amry, E.A. Baker, L.S. Suwandi, M.D. McCain, M.F. Rahman, S. Dickey, and D.V. Lim. 2003. Effect of aryl ring fluorination on the antibacterial properties of C4 aryl-substituted N-methylthio ß-lactams. Bioorg. Med. Chem. 11:1859-1863.
Lim, D.V. 2003. Detection of microorganisms and toxins with evanescent wave, fiber optic biosensors. Proc. IEEE. 91: 902-907.
Tims, T.B. and D.V. Lim. 2003. Confirmation of viable E. coli O157:H7 by enrichment and PCR after rapid biosensor detection. J. Microbiol. Meth. 55:141-147.
Kramer, M.F. and D.V. Lim. 2004. A rapid and automated fiber optic-based biosensor assay for the detection of Salmonella in spent irrigation water used in the sprouting of sprout seed. J. Food Protect. 67:46-52.
Harwood, V.J., N.C. Delahoya, R.M. Ulrich, M.F. Kramer, J.E. Whitlock, J.R. Garey, and D.V. Lim. 2004. Molecular confirmation of Enterococcus faecalis and E. faecium from clinical, fecal and environmental sources. Letts. Appl. Microbiol. 38:476-482.
Donaldson, K.A., M.F. Kramer, and D.V. Lim. 2004. A rapid detection method for Vaccinia virus, the surrogate for smallpox virus. Biosensors and Bioelectronics 20:322-327.
Tims, T.B. and D.V. Lim. 2004. Rapid detection of Bacillus anthracis spores directly from powders with an evanescent wave fiber-optic biosensor. J. Microbiol. Methods 59:127-130.
Nyquist-Battie, C., L.E. Frank, D. Lund, and D.V. Lim. 2004. Optimization of enzyme-linked immunosorbent assay detection of Escherichia coli O157:H7 in apple juice. J. Food Protection 67:2756-2759.
Liam, W., S.A. Litherland, H. Badrane, W. Tang, D. Wu, H.V. Baker, P.A. Gulig, D.V. Lim, and S. Jin. 2004. Ultra-sensitive detection of biomolecules with fluorescent dye-doped nanoparticles. Anal. Biochem. 334:135-144.
Simpson, J.M. and D.V. Lim. Rapid PCR Confirmation of E. coli O157:H7 after evanescent wave fiber optic biosensor detection. 2005. Biosensors and Bioelectronics 21:881-887
Nyquist-Battie, C., L. Mathias, L.E. Frank, D. Lund , and D.V. Lim. 2005. Antibody-based detection of acid-shocked, acid-adapted, and apple juice-incubated Escherichia coli O157:H7. J. Immunoassay and Immunochemistry 26:259-271.
Turos, E., C. Coates, J-Y Shim, Y. Wang, J.M. Leslie, T.E. Long, G.S.K. Reddy, A. Ortiz, M. Culbreath, S. Dickey, D.V. Lim, E. Alonso, and J. Gonzalez. 2005. N -Methylthio ß-lactam antibacterials: effects of the C 3 /C 4 ring substituents on anti-MRSA a ctivity. Bioorg. Med. Chem. 13:6289-2308.
Lim, D.V., J.M. Simpson, E.A. Kearns, and M.F. Kramer. 2005. Monitoring for agents of bioterrorism/biowarfare. Clin. Microbiol. Rev. 18:583-607.
Turos, E., R.K. Mishra, K.D. Revell, C.M. Coates, S. Dickey, and D.V. Lim. 2006. N -Thiolated 2-oxazolidinones: a new family of antibacterial agents for methicillin-resistant Staphylococcus aureus (MRSA) and Bacillus anthracis. Bioorg. Med. Chem. Lett. 16:2081-2083.
Turos, E., T.E. Long, B. Heldreth, J.M. Leslie, S.K.R. Guntireddygari, Y. Wang, C. Coates, M. Konaklieva, S. Dickey, D.V Lim, E. Alonso, and J. Gonzalez. 2006. N- Thiolated ß -lactams: a new family of anti- Bacillus agents. Bioorg. Med. Chem. Lett. 16:2084-2090.
Heldreth, B., T.E. Long, S. Jang, G.S.K. Reddy, E. Turos, S. Dickey, and D. V. Lim. 2006. N-Thiolated ß-lactam antibacterials: effects of the N-organothio substituent on anti-MRSA activity. Bioorg. Med. Chem. 14:3775-3784.
Turos, E., J-Y. Shim, Y. Wang, K. Greenhalgh, G.S.K. Reddy, S. Dickey, and D.V. Lim. 2007. Antibiotic-conjugated polyacrylate nanoparticles: new opportunities for development of anti-MRSA agents. Bioorg. Med. Chem. Lett. 17:53-56.
Turos, E., S.K. Reddy, K. Greenhalgh, S.A. Abeylath, S. Dickey, and D.V. Lim. 2007. Penicillin-bound polyacrylate nanoparticles: restoring the activity of beta-lactam antibiotics against MRSA. Biorg. Med. Chem. Lett. 17:3468-3472.
Nyquist-Battie, C., L. Freeman, J.P.S. Soberano, D. Lund, E. Kearns, and D.V. Lim. 2007. Effects of chlorine injury, starvation and Colitag enrichment on sandwich enzyme-linked immunosorbent assay (sELISA) detection of Escherichia coli O157:H7. J. Immunoassay Immunochem. 28:371-383.
Kramer, M.F., G. Vesey, N.L Look, B.R. Herbert, J.M. Simpson and D.V. Lim. 2007. Development of a Cryptosporidium oocyst assay using an automated fiber optic-based biosensor. J. Biol. Eng. 1:3.
Simpson-Stroot, J.M., E.A. Kearns, P.G. Stroot, S. Magaña, and D.V. Lim. 2008. Monitoring biosensor capture efficiencies: development of a model using GFP-expressing Escherichia coli O157:H7. J. Microbiol. Meth. 72:29-37.
Abeylath, S.C., E. Turos, S. Dickey, and D.V. Lim. 2008. Glyconanobiotics: Novel carbohydrated nanoparticle antibiotics for MRSA and Bacillus anthracis. Bioorg. Med. Chem. 16:2412-2418.
Turos, E., K.D. Revell, P. Ramaraju, D.A. Gergeres, K. Greenhalgh, A. Young, N. Sathyanarayan, S. Dickey, D.V. Lim, M.M. Alhamadsheh, K. Reynolds. 2008. Unsymmetric aryl-alkyl disulfide growth inhibitors of methicillin-resistant Staphylococcus aureus and Bacillus anthracis. Bioorg. Med. Chem. 16:6501-6508.
Brown, D.R., L.D. Wednland, G.J. Ortiz, M.F. Kramer, D.V. Lim, M.B. Brown, and P.A. Klein. 2008. Evanescent-wave biosensor for field serodiagnosis of tortoise mycoplasmosis. Vet. Immunol. Immunopathol. 124:322-331.
Kearns, E.A., S. Magana, and D.V. Lim. 2008. Automated concentration and recovery of microorganisms from drinking water using dead-end ultrafiltration. J. Appl. Microbiol. 105:432-442.
Leskinen, S.D. and D.V. Lim. 2008. Rapid ultrafiltration concentration and biosensor detection of enterococci from large volumes of Florida recreational water. Appl. Environ. Microbiol. 74:4792-4798.
Nyquist-Battie, C., L. Freeman, K. Leckband, S. Martinez, A. Ansley, D. Lund, and D.V. Lim. 2008. Antibody-based detection of Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium grown in low-shear modeled microgravity. Micrograv. Sci. Technol. 20:23-28.
O’Driscoll, M., K. Greenhalgh, A. Young, E. Turos, S. Dickey, and D.V. Lim. 2008. Studies on the antifungal properties of N-thiolated beta-lactams. Bioorg Med. Chem. 16:7832-7837.
Denton, K.A., M.F. Kramer, and D.V. Lim. 2008. Rapid detection of Mycobacterium tuberculosis in lung tissue using a fiber optic biosensor. J. Rapid Methods and Automation in Microbiology 16:439-453.